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发表于 2013-10-4 15:01:35
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canada goose jacket I can't do a monstrous face
I can't do a massive face
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succeeding protein transfer (Total cellular extracts from keratinocytes mixed with small amounts of [35S]Methioninelabeled removes) to nitrocellulose, The sheet or strip was dried and exposed to an Xray film. The amount of keratinocyte cell extract applied to the gels was carefully calibrated to obtain the maximum amount of protein transferred without affecting the quality of the 2D gels. Dry sheets could be kept for extended time (about 3 mo) Without considerable change in the reactivity of the proteins.
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